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By M. deBroe, et al.

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Sterile conditions for culture. Cell lines Easily obtained and subcultured. Origin ill-defined. Important dedifferentiation. Primary cultures Closely related to fresh tissue. Origin identified. More difficult to prepare and maintain. Vesicles Transport in apical and basolateral membranes can be studied separately. No metabolism. No intracellular sequestration. Membrane isolation may alter physiological function. Must correct for non-mediated transport. Adapted from Williams & Rush [6] and Brater et al [8].

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