Download Advances in Clinical Chemistry, Vol. 19 by Oscar; Latner, A. L. Bodansky PDF

By Oscar; Latner, A. L. Bodansky

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The photometer cuvette is constructed of silver, to accelerate temperature equilibration, and has a path length of 1 cm. The volume of solution 44 D. W. 19. (A) The Perkin-Elmer KA-150 Kinetic Analyzer. (B) Diagram of operational sequence. ) required to fill it is 20 pl. Air-segmentation of the reaction mixture flowing through the cuvette is used to reduce carry-over from sample to sample. OS"C. Reaction mixtures corresponding to each sample reach the photometer cell 25 seconds after the reaction has been triggered by substrate.

The transfer disk is removed from the rotor and loaded with measured volumes of samples and reagents, either manually or in an automatic pipetting and dispensing module. After replacing the loaded transfer disk in the center of the cuvette ring the assembly is accelerated rapidly to a rotational speed of about 1000 rpm in a few seconds. The resulting centrif- 30 D. W. MOSS Siphon channel Cuveffe rotor Transtel’ disk Sample and reagent w e f h Cuvetfe chamber I L ighf path FIG. 13. Diagrammatic cross section of the rotor of a parallel fast analyzer showing ariangement of sample and reagent wells and cuvettes.

5 ml is necessary to ensure complete washing of the transfer tubing and the cuvette. Arrival of a reaction vessel at the transfer station also triggers the autozero mechanism of the spectrophotometer. This offsets the initial absorbance of the reaction mixture and ensures that all traces begin from zero on the chart recorder, if this is in use. Computations of catalytic concentration are made by multiplying the absorbance change registered over 30 or 60 seconds by the appropriate factor, previously entered into the calculator.

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